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Operating HEF® on see p. Tab. Fig. —p.
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Copyright Copyright© 2011 New Brunswick Scientific Co., Inc., USA. No part of this publication may be reproduced without the prior permission of the copyright owner. New Brunswick Scientific reserves the right to change information in this document without notice. Updates to information in this document reflect our commitment to continuing product development and improvement. Trademarks Eppendorf® is a registered trademark of Eppendorf AG, Germany.
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HEF® and G -86 °C Freezers — Operating manual 1 Table of contents 1 Operating instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 1.1 1.2 1.3 1.4 2 3.3 3.4 3.5 4.3 4.4 4.5 Main illustration. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 Delivery package . . . . . . . . . . . . . . . . . . . . . . . .
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HEF® and G -86 °C Freezers — Operating manual 1 Table of contents 6.3 6.4 7 7.2 Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7.1.1 Painted surfaces . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7.1.2 Panels and shelves . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
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HEF® and G -86 °C Freezers — Operating manual 1 Operating instructions 1 1.1 Operating instructions Using this manual 1 Carefully read this operating manual before using the device for the first time. Also observe the operating manual enclosed with the accessories. The operating manual should be considered as part of the product and stored in a location that is easily accessible. Operating instructions When passing the device on to third parties, be sure to include this operating manual.
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HEF® and G -86 °C Freezers — Operating manual 1.
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HEF® and G -86 °C Freezers — Operating manual 2 Safety 2 Safety 2.1 Intended use New Brunswick HEF and G (Green) freezers are designed to provide precise, ultra-low temperature environments for cold storage of scientific or medical materials. They are designed to provide ultra-low temperature sample storage from -50 °C to -86 °C at 32 °C maximum ambient operating temperature. 2.2 Warnings for intended use 2.2.
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HEF® and G -86 °C Freezers — Operating manual 6. The hydrocarbon (Group A3) refrigerants used in these freezers are flammable and therefore appropriate attention must be paid to avoid leaks and to keep the freezer away from sparks and flames.
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HEF® and G -86 °C Freezers — Operating manual 3 Product description 3 Product description 3.1 Main illustration Abb. 1: Upright freezer - Side and front views 1 2 3 3 Product description 4 5 11 Fig.
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HEF® and G -86 °C Freezers — Operating manual Abb. 2: Chest freezer - Front view 2 3 1 4 5 6 7 3 Product description 8 9 Fig. 2: Chest freezer - Front view 1 Heated vent port (inside compressor housing) 2 Lockable handle 3 Control panel/display 4 Battery switch (alarm) behind lockable panel 5 On/Off circuit breaker behind lockable panel 6 Chart recorder (optional) 7 Quarter turn fasteners 8 Air filter grille 9 Braked castors (front) 3.2 Delivery package 3.2.
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HEF® and G -86 °C Freezers — Operating manual 3.3 Product versions 3.3.1 Introduction This manual provides the user with the necessary information for installation and operation of New Brunswick’s energy saving HEF and G range of Ultra-Low Temperature freezers: Innova® U725G, and the HEF models. It also provides some preliminary user maintenance information. This manual covers the following freezer models: Model (230 V, 50 Hz) Capacity Innova U725-G Air-cooled 725 liters (25.
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HEF® and G -86 °C Freezers — Operating manual • A heated port with ice-clearing plunger prevents vacuum formation enabling the outer door to be easily opened at anytime. • All interior panels and shelves are made of high grade corrosion-resistant stainless steel, making them durable and easy to keep clean and sterilize. • Heavy duty castors enable easy installation and relocation.
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HEF® and G -86 °C Freezers — Operating manual 4 Installation 4 Installation 4.1 Preparing installation Risk of personal injury DO NOT attempt to lift any freezer by hand. Preferred lifting for loading and unloading is by WARNING! mechanical lifting equipment. Risk of material damage Maintenance, adjustment and repair work should be carried out only by QUALIFIED, NOTICE! EXPERIENCED personnel who have been AUTHORIZED to undertake such work by New Brunswick Scientific or its authorized agents.
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HEF® and G -86 °C Freezers — Operating manual 4.2.3 Installation categories (230 V, 50 Hz models only) Category A1 (domestic/ public) Examples Requirements Hospitals < 0.15 kg refrigerant per sealed system Prisons Theatres Schools Supermarkets Hotels Dwellings B1 (commercial/ private) Business or professional offices < 0.15 kg refrigerant per sealed system Shops Restaurants Laboratories General manufacturing 4.
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HEF® and G -86 °C Freezers — Operating manual 4.4 Installing the shelves Model U410 HEF and U570 HEF freezers are fitted with four adjustable shelves. Model U725 freezers are fitted with two adjustable shelves. These can be positioned in 12.7 mm (½ in) steps anywhere throughout the freezer. To effectively utilize racks within the freezer, be sure to position them so that each shelf is aligned with the bottom of each inner door. Perform the following steps to install the shelves: 1.
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HEF® and G -86 °C Freezers — Operating manual 2. Remove the two screws from behind the lock barrel. 1 2 3 4 Installation 4 1 Freezer handle 2 Screw (1 of 2) 3 Lining plate 4 Freezer door wall 3. Remove the lining plate and lock barrel. 4. Insert the plastic blanking plug supplied. 5. Insert the lining plate and screw in the two screws. It is important that the handle lock lining plate be installed at all times.
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HEF® and G -86 °C Freezers — Operating manual 5 5 5 Operating controls and function Controls and function Operating controls and function 5.1 Operating controls and function Operating controls are located on a control panel mounted in the door of the upright freezers, and on the console on the right side of chest freezers. Every New Brunswick freezer is equipped with S.M.A.R.T. Plus™ diagnostic software, to help identify the cause of a fault or setpoint variance.
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HEF® and G -86 °C Freezers — Operating manual 5 Operating controls and function Temp-alarm light Item Name Function 8 TEMP-ALARM Should a mains/power failure cause the temperature to surpass the alarm setpoint, the TEMP-ALARM illuminates. (The audible alarm will already be sounding due to the mains/power failure). The TEMP-ALARM lamp will extinguish when the temperature returns to normal set range. Cancel the TEMP-ALARM by pressing the ALARM TEST/MUTE KEY.
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HEF® and G -86 °C Freezers — Operating manual 5 The keypad controls are locked when the freezer is under remote control. Hint! Operating controls and function Set temp key Operation in normal mode with LOCK lamp off. Item Name 7 SET TEMP Function Displays current temperature setting. Used to change temperature settings. High alarm/low alarm keys Operation in normal mode with LOCK lamp off. Item Name Function 2 HIGH-ALARM Displays current high alarm temperature setting.
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HEF® and G -86 °C Freezers — Operating manual 5 "C" key Operating controls and function Operation in normal mode with LOCK lamp off. Item Name 14 C Function Used to cancel data when programming. Numerical keys Operation in normal mode with LOCK lamp off. 20 Item Name 15 NUMERICAL KEYS (1-0) Function Used to input data when programming. Keys 8 and 9 are also used to program alarm delays (see Setting the alarm delay on p. 25).
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HEF® and G -86 °C Freezers — Operating manual 6 Operation 6 Operation 6.1 Getting started 6 Risk of personal injury BEFORE connecting the freezer to the mains/electrical supply, make sure that the mains/ WARNING! Operation 6.1.1 power supply matches the requirements of the equipment. Check the specification plate (located on the side of the freezer) for the electrical requirements. The equipment should be connected to an earthed/grounded outlet socket.
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HEF® and G -86 °C Freezers — Operating manual Abb. 4: Upright freezers switch location 6 1 Operation 2 Fig. 4: 1 Upright freezers switch location Battery switch Abb. 5: 2 On/Off circuit breaker switch Chest freezers switch location 1 2 Fig. 5: 1 Chest freezers switch location Battery switch Hint! 6.1.
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HEF® and G -86 °C Freezers — Operating manual If the freezer is turned off during the initial pull-down period, the alarm will activate 30 minutes after switching it back on. The factory-set temperature is -80°C. 6.1.4 Testing the alarm monitoring socket 6 The freezer is fitted with a remote alarm socket for testing power-fail and low battery alarms, and for connection to an external building monitoring system or optional auto-dialer, (see Alarm monitoring socket on p. 26).
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HEF® and G -86 °C Freezers — Operating manual 6.2.2 Setting high alarm setpoint The high alarm setpoint may not be warmer than -10 °C and may not be less than within +5° of the operating temperature. The default setting is +5° from the temperature setpoint. 1. Press the LOCK key. 6 Hint! The LOCK lamp will flash if a lock code (password protection) is required, (see Changing lock codes on p. 25). Operation The LOCK lamp will illuminate, indicating the system is unlocked and parameters can be changed.
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HEF® and G -86 °C Freezers — Operating manual 6.2.4 Checking temperature and alarm setpoint settings To view the currently set operating temperature, high alarm setpoint, or low alarm setpoint for the freezer. Press the SET TEMP key, HIGH ALARM key, or the LOW ALARM key and read the display. Hint! Setting the alarm delay Operation 6.2.
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HEF® and G -86 °C Freezers — Operating manual The freezer is delivered unlocked. To change the code, the freezer must be unlocked. If a lock code has already been set (indicated by the LOCK lamp flashing when the LOCK key is pressed), that same code must be entered to unlock the freezer. When the freezer is unlocked, the LOCK lamp is on (not flashing). Once the freezer is unlocked, follow these steps to set a new lock code: 6 1. Press the CODE CHANGE key. The lamp will flash and the display will go blank.
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HEF® and G -86 °C Freezers — Operating manual Abb. 6: Upright freezers alarm monitoring socket 6 1 2 Fig. 6: Upright freezers alarm monitoring socket 1 RS-485 connector (optional) 3 Mains/power socket Abb. 7: Operation 3 2 Alarm monitoring socket 2 Alarm monitoring socket Chest freezers alarm monitoring socket 1 Fig. 7: 2 3 Chest freezers alarm monitoring socket 1 RS-485 connector (optional) 3 Mains/power socket The configuration of the socket is shown in (Fig. 8 on p.
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HEF® and G -86 °C Freezers — Operating manual Abb. 8: Remote alarm socket - upright freezer Operation 6 28 Fig. 8: Remote alarm socket - upright freezer Abb. 9: Remote alarm socket - chest freezer Fig.
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HEF® and G -86 °C Freezers — Operating manual 7 7 7.1 Maintenance Maintenance Cleaning Risk of material damage Maintenance, adjustment and repair work should be carried out only by QUALIFIED, EXPERIENCED personnel who have been AUTHORIZED to undertake such work by New Brunswick Scientific or its authorized agents. NOTICE! Failure to use authorized service agents will invalidate the warranty. 7.1.1 7 Painted surfaces 7.1.
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HEF® and G -86 °C Freezers — Operating manual 1 2 3 Maintenance 7 7.1.5 1 Plunger 3 Freezer outer wall 2 Cover Door or lid seal Be sure to treat the door or lid seal with care. Avoid damaging this seal in any way. The freezer cannot operate properly with a defective seal. It is advisable to wipe both the seal and the surface against which it seals with a soft dry cloth once a month. 7.2 Routine maintenance 7.2.
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HEF® and G -86 °C Freezers — Operating manual 3. Remove the inner door and set aside. Abb. 10: Screw hinge inner door 1 2 7 1 Maintenance Fig. 10: Screw hinge inner door Inner door 2 Door hinge screw x4 Repeat procedure for each door. 7.2.4 Replacing the inner door (upright freezers except U725-G) To reinstall the inner doors of the upright freezers (except U725-G): 1. Fully open the outer door of the freezer. 2. Reposition inner door in closed position. 3. Screw on door hinges. 4.
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HEF® and G -86 °C Freezers — Operating manual 7.2.5 Removing the inner doors (U725-G) To remove the inner doors of the U725-G freezer: 1. Fully open the outer door of the Freezer. 2. Fully open the inner door. 3. Lift off inner door from hinges and set aside. Abb. 11: Lift-off inner door 1 2 Maintenance 7 Fig. 11: 1 Lift-off inner door Inner door 2 Lift-off hinge Repeat procedure for each door. 7.2.6 Replacing the inner door (U725-G) To replace the inner door of the U725-G freezer: 1.
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HEF® and G -86 °C Freezers — Operating manual 7.2.7 Electrical components Risk of personal injury All electrical components that could cause possible ignition of refrigerant vapor during normal WARNING! operation have been enclosed in an IP65 enclosure. During routine maintenance, care must be taken to avoid any damage to the gaskets and sealing grommets of these enclosures; also check the gaskets and sealing grommets routinely to ensure their integrity.
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HEF® and G -86 °C Freezers — Operating manual 8 8 8.1 Troubleshooting Troubleshooting General errors If you are experiencing a problem with your freezer, check the following troubleshooting guides before contacting your New Brunswick authorized Service technician. Symptom/ message Door will not open Cause Remedy 1. The door handle is locked. 1. Unlock the door handle. 2. The heated vent port is blocked. 2. Break up the ice in the vent port using the plunger, (see Heated vent port on p. 29).
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HEF® and G -86 °C Freezers — Operating manual 8.3 After a mains/power failure If mains/power is interrupted, the POWER-FAIL indicator lamp (see Fig. 3 on p. 17), Item 3, will illuminate. In addition, the audible alarm will sound and the display will flash at approximately 10-second intervals. When mains/power is restored, both alarm and light will automatically be cancelled.
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HEF® and G -86 °C Freezers — Operating manual 9 Technical data 9 Technical data 9.0.1 Specifications 9.0.2 Specifications for U725-G Air-cooled and U725-G Water-cooled freezers Model No. U725-G Air-cooled U725-G Water-cooled U9440-0005 U9440-0004 1365 x 865 x 615 mm 1365 x 865 x 615 mm 53.7 x 34.0 x 24.2 in 53.7 x 34.0 x 24.2 in 1950 x 1025 x 867 mm 1950 x 1025 x 867 mm 76.8 x 40.4 x 34.1 in 76.8 x 40.4 x 34.1 in 725 Liters 725 Liters 25.6 cubic feet 25.
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HEF® and G -86 °C Freezers — Operating manual 9.0.3 Specifications for U410 HEF and U570 HEF freezers Model No. U410 HEF U570 HEF U9260-0008 U9260-0007 U9270-0008 U9270-0007 1265 x 550 x 575 mm 1265 x 765 x 575 mm Height x Width x Depth 49.8 x 21.6 x 22.6 in 49.8 x 30.1 x 22.6 in External Dimensions: 1915 x 800 x 852 mm 1925 x 1025 x 852 mm 75.3 x 31.5 x 33.5 in 75.8 x 40.3 x 33.5 in Part No. Internal Dimensions: Height x Width x Depth Capacity Net Weight Lock No.
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HEF® and G -86 °C Freezers — Operating manual 9.0.4 Specifications for C660 HEF freezers Model No. C660 HEF Part No. U9250-0008 U9250-0009 Internal Dimensions: 760 x 1470 x 590 mm Height x Width x Depth 30 x 57.8 x 23.2 in External Dimensions: 1075 x 2050 x 840 mm Height x Width x Depth 42.3 x 80.7 x 33 in Capacity 660 Liters 23.3 cubic feet Net Weight 304 kg 669 lb Lock Standard No.
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HEF® and G -86 °C Freezers — Operating manual 10 10 10.1 Ordering Information Ordering Information Accessories A number of accessories are available for New Brunswick's range of ultra-low temperature freezers. Contact your local New Brunswick representative or distributor for details. 10.1.
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HEF® and G -86 °C Freezers — Operating manual 10 10.1.9 New Brunswick BioCommand SFI datalogging software (RS-485 interface) Ordering Information New Brunswick Scientific's BioCommand® SFI Track and Trend software is available to trend and archive data from as many as 32 shakers, CO2 incubators, and/or freezers, simultaneously. This PC-based software works with any OPC-compliant equipment with an RS-232 or RS-485 port.
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HEF® and G -86 °C Freezers — Operating manual 11 11 11 Transport, storage and disposal Transport, storage and disposal Shut down Transport, storage and disposal 11.1 Switch the battery switch off before transporting or storing the equipment, (see Battery backup switch on p. 26). 11.2 Transport Risk of personal injury WARNING! Lifting and transporting the freezer without suitable equipments can result in crushing and other injuries.
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HEF® and G -86 °C Freezers — Operating manual 12 12 Certificates Certificates 12 Certificates DECLARATION OF CONFORMITY New Brunswick Scientific declares that the Ultra Low Temperature Freezers products listed conform to the European Union Directives and Standards identified in the declaration.
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HEF® and G -86 °C Freezers — Operating manual HCFC................................................................................. 6 Index Index A Accessories .....................................................................39 Adjusting the shelf ...........................................................15 Air intake ..........................................................................29 Alarm ...............................................................................26 Alarm activation ..
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HEF® and G -86 °C Freezers — Operating manual RS-485 computer interface ..............................................40 S S.M.A.R.T. Plus..........................................................17, 34 Safety.................................................................................7 Set temp key ....................................................................23 Setpoint, temperature ......................................................35 Setting the operating temperature ......................
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New Brunswick Scientific • www.nbsc.com • bioinfo@nbsc.com • For your local sales office or distributor contact: Eppendorf AG · 22331 Hamburg · Germany · Tel: +49 40 538 01-0 · Fax: +49 40 538 01-556 · E-mail: eppendorf@eppendorf.com New Brunswick Scientific Europe B.V. · Nijmegen · The Netherlands · Tel: +31 (0) 24 3717 600 · Email: europe@nbsbv.nl Eppendorf North America, Inc. · Hauppauge, N.Y. USA · Tel: +1 516 334 7500 · +1 800 645 3050 · E-mail: info@eppendorf.
